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We narrowed to 795 results for: Tec

Showing: 461 - 480 of 795 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...repair of the break to allow for site-specific detection downstream. The con to these approaches is that...that NHEJ must perform the repair to allow for detection, not Homologous Recombination or Alternative End...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    .... Alternatively, you can perform simultaneous detection with a mixture of primary antibodies and appropriate..., M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex...Introduction to Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... Why might one be interested in state machine technology? Biological research has produced a massive amount...itself to cheap, quick, and high throughput state detection with flow cytometry. However, expressing reporters...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

  5. Finding Your Perfect Job After University

    Type
    Blog Post
    ...developing an easy to use diagnostic tool for the detection and diagnosis of different types of pancreatic...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

  6. Antibodies 101: Validation

    Type
    Blog Post
    ... good correlation between the levels of target detected in both methods. These approaches have relatively...are ultimately planning to use your antibody to detect endogenous levels of the target in other cells ...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    .... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...even if they are present in the sample. Other techniques, like electron microscopy and FRET, can offer...details are still being uncovered, and different techniques all bring their own advantages—and blind spots—to...RNA-programmed Cas9 nuclease specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt...

  9. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ... looking for the best way to improve your lab techniques — but perhaps are a little limited on time — ...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...

  11. CRISPR Guide Update

    Type
    Blog Post
    ...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ... at MIT. He also writes Codon, a blog about biotechnology.    ...

  14. Call for Guest Bloggers

    Type
    Blog Post
    ...recent advances in the biological sciences and techniques that simplify and expedite research. We recognize...through our blog. Do you have skill in a particular technique? Do you have a set of tips you give to every new...

  15. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

  16. Summer Fun at Addgene!

    Type
    Blog Post
    ... with our 101 series, which introduces common techniques and concepts. But what if you’re looking for ...Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head...

  18. Editor's Choice, October 2016

    Type
    Blog Post
    ...ways to use CRISPR in plants, to learn techniques for detecting genome edits, to discover why you might...

  19. Editor's Choice, July 2016

    Type
    Blog Post
    ...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...
Showing: 461 - 480 of 795 results