We narrowed to 501 results for: Trac;

1. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   ... promise! FSC and SSC: scattering To digitally extract your cells based on their size, the cytometer provides...

2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...labeled ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the ...

3. Tips for Getting a Faculty Position

   Type

   Blog Post

   ...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...

4. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...

5. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   ...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...expression of the transgene. This makes IDLVs an attractive choice for certain types of clinical or basic...

7. 10 Steps to a Perfect Science Talk

   Type

   Blog Post

   ...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...

8. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   ...efficiency of the dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species...

9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ...problem of in vivo CRISPR delivery become more tractable in the next few years, as it would open up many...

10. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ... cloning) and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several ...

11. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    ...14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs, still...

12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ... characterised antibodies against all human extracellular proteins with community-based validation. They...

13. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    ... also be a sign that the lab head has trouble attracting scientists. To get a better idea of why the lab...

14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    ...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...

15. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    ...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...

16. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ... am not sure that I want to pursue the academic track.” How does an International Mentorship Program work...

17. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    ... and how important is the result? Is there a tractable question or are you simply describing a phenomenon...

18. Finding Your Science Policy Path

    Type

    Blog Post

    ...for skill development include leadership of extracurricular organizations, working in student government...

19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ... single-nucleotide substitutions along a 1.5 kb tract, all without selectable markers.  CRISPR in C. elegans...

20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    ... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...