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We narrowed to 750 results for: Uty

Showing: 461 - 480 of 750 results

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    .... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...

  7. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotinylating your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining ...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can...What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time to consider the...

  13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...next few years, as it would open up many new therapeutic possibilities for some of the world’s most common...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9...

  16. A Control for All Seasons

    Type
    Blog Post
    ...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...them? Or maybe you just want to make some really beautiful images? Multiplex IF may be just what you need... L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific...that also express GAL4. Usage of this system revolutionized genetic studies in flies. An additional component...

  20. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... percentage of acrylamide. To further improve resolution, use a stacking gel on top of the resolving gel...Gradient gels are another great option to enhance resolution. In this setup, gels have an increasing range...
Showing: 461 - 480 of 750 results