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We narrowed to 771 results for: Vars

Showing: 461 - 480 of 771 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience...

  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  8. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  10. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  11. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  12. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...reactions that work in just about any situation (Devaraj & Finn, 2021). In one of the most common Click ...clicking. References and Resources References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click...

  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  17. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  18. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...
Showing: 461 - 480 of 771 results