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Showing: 461 - 480 of 767 results

  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience...

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ...supply of transgenic reporter lines that supply a variety of GFP expression patterns, thus allowing you to...

  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

  6. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...plasmids can direct the production of virus with various tropisms.  Packaging using helper-free packaging... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...

  8. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

  9. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  10. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  13. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018)....

  16. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  20. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...
Showing: 461 - 480 of 767 results