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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 773 results for: Vars;

Showing: 461 - 480 of 773 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018)....

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new...

  5. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  11. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  13. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  14. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  17. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  20. A Look at Addgene's QC Process

    Type
    Blog Post
    ...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...
Showing: 461 - 480 of 773 results