We narrowed to 772 results for: Vars;
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TypeBlog Post... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...
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A Deep Dive into BioBuilder
TypeBlog Post...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018).... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post... available. We’ve also had some requests for variations on the plasmids we’ve developed. We’re working...be doing something similar. There may be other variants of this that are better for some experiments than... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...the degree to which genes are turned on and off varies depending on which guide RNA we use. What we have...straightforward to apply the new library for use in various cell types and in live mice. Therapeutic uses in... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post...reactions that work in just about any situation (Devaraj & Finn, 2021). In one of the most common Click ...clicking. References and Resources References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click... -
Summer SciComm Series: Cognitive Load
TypeBlog Post... Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts. How do you develop problem solving... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...is going to vary widely. The way you communicate your science at a poster is going to vary depending on... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible... -
RNA Extraction Without A Kit
TypeBlog Post...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...interest. Thanks to the tremendous efforts made by various research teams to build and constantly improve ...integrated, we look forward to the realization of a variety of perturbations and applications in functional... -
A Look at Addgene's QC Process
TypeBlog Post...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited... -
Hot Plasmids - October 2020
TypeBlog Post...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...CRISPR activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes...factors to increase gene expression. CRISPRa methods vary in their transcriptional activators: some methods... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing...out this post. Learn how to Choose the Best Cas9 Variant for Your Next Experiment Read more about Optogenetic... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...