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We narrowed to 1,091 results for: addgene

Showing: 461 - 480 of 1091 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody... combines a marker with a signal. For clarity, Addgene uses 'labeling' to refer to antibodies and 'staining...Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect detection of target...Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot Antibodies...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...pAAV-mDlx-GCaMP6f-Fishell-2  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated ... Vectors for Blue White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin at the beginning...which rescue a lethal phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains...

  7. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own ...details on the production process, check out the Addgene AAV Production protocol. Titering Once you’ve produced...details on the qPCR titering process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested...interested in the ddPCR titering process, which is what Addgene uses, check out our ddPCR for AAV quantitation ...10.1089/1043034041361262 More resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of...PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression...deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes in a variety..., MA: Cell Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard. However,...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...PMCID: PMC5167268. Additional resources on the Addgene blog Read more about polymerase chain reaction... vector related protocols Learn more about the Addgene viral service ...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned...lentiviral packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles ...plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR v2,... terms of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral...PubMed PMID: 19847206. Additional Resources on the Addgene Blog Your Lentiviral Plasmid FAQs Answered Learn...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus...

  13. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...resources by sharing plasmids Find plasmids at Addgene ...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...

  15. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

  16. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ... Various Addgenies...

  17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...various plasmids for overexpression and knockdown. Addgene also has a number of insect vectors to consider...easy way to see what is available is to browse Addgene’s Collection under the insect expression system ...species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly ...PMCID: PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read ...for Site-Specific Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols...

  19. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...Immunotherapies   Resources on Addgene.org Fine More Kits at Addgene Browse Plasmids from the Broad ... available to the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA...and we’re proud to have worked closely with our Addgene colleagues to make these available to the scientific...Twitter @boehmjesse.   Additional Resources on the Addgene Blog Learn About our Cancer Colleciton Pages Use...

  20. Summer SciComm: Preprints

    Type
    Blog Post
    ...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly — which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...
Showing: 461 - 480 of 1091 results