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Showing: 461 - 480 of 1095 results
  1. Choosing Your Perfect Empty Backbone

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    ...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ...
  2. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post
    ...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Reproducibility at Protocols.io Additional Resources on the Addgene Blog FPbase: A new community-editable fluorescent...
  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post
    ...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to...
  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process... the plasmid sequences). As with all things at Addgene, each part of this new process involves a significant...available as a partial sequence with the heading "Addgene Partial NGS Result." In some cases, there will ...
  5. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...1 are available at Addgene. Table 1: List of pCXLE episomal plasmids, their Addgene ID numbers and the...Field Sign Up to Write for the Addgene Blog! Resources on Addgene.org Browse Our Stem Cell Collection... diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells have been ...reprogramming factors they encode Episomal Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL...PMCID: PMC2922531. Additional Resources on the Addgene Blog Stem Cell Models for Disease & Open Science...
  6. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post
    ...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post...
  7. Bringing Sustainable Practices to the Lab: Easy Action Items

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    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...resources by sharing plasmids Find plasmids at Addgene ...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...
  8. Grad School Advice Part 2: Building Community

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...
  9. Grad School Advice Part 1: Picking a Lab and a Project

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...
  10. Fueled by Coffee at #SfN14

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    Blog Post
    ... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...by booth #3431 if you’re at #SfN14 and meet our Addgenie crew and sign-up for a free plasmid! Also check...
  11. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...
  12. Plasmids 101: The Wide World of Natural Plasmids

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    ...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy...deposit the many plasmid tools available in the Addgene repository. To learn more about some other common...other Plasmids 101 posts, and keep an eye on the Addgene blog as scientists continue to explore Mother Nature.../07388550490904288 Additional Resources on the Addgene Blog Plasmids 101: Antibiotic Resistance Genes...
  13. Cultivating Community Science at BosLab

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    ... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...
  14. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ... Various Addgenies...
  15. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ...and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...interest.   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python....ymthe.2017.04.001 Additional resources on the Addgene blog Read all of our viral vectors related blog...
  16. Viral Vectors 101: Transductions

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    Blog Post
    ...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...
  17. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered...annotation gaps in the plasmids available through the Addgene website. In addition, two initiatives are in the...University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he needed it for...post-launch iterations.     Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...
  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog An “elegans” Approach...PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9 editing in ...PubMed. Find plasmids from this publication at Addgene. Scalable and versatile genome editing using ...
  19. Hot Plasmids: FRET-Based Biosensors

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    ...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging...
Showing: 461 - 480 of 1095 results