We narrowed to 592 results for: arl

1. Hot Plasmids and Viral Preps - January 2021

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   ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ...and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess the effect of removing...

3. Corralling and Cornering the Goldilocks Antibody

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   ...from the background noise. If the sample images similarly to the negative control, that informs us that ...

4. Viral Vectors 101: Virus Safety

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   ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...

5. Antibodies 101: Choosing the Right Isotype

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   ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

6. Hot Plasmids February 2024

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   ...Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases) and minimal toxicity, including...

7. Viral Vectors 101: Calcium Sensors

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   ... aims and requirements of in vivo studies. A particularly useful sensor for neuroscientists is CaMPARI...

8. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...rules the best way to design GGA overhang sets, particularly for high-complexity reactions? The researchers...

9. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   ...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...

11. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

12. 6 Tips for Grant Writing

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    Blog Post

    ...increase your success rate. 1. Submit your grant early! First off, and perhaps the most important bit of...

13. Cpf1 Update: Comparison to Cas9 and NgAgo

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    .... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...

14. Plasmids 101: Terminators and PolyA signals

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    ...additional references are provided at the end.  Nearly all common bacterial expression plasmids use Rho-independent...

15. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...serial dilutions of a viral prep. This method is particularly useful when vectors carrying fluorescent markers...

16. Illuminating Epigenetics with A FRET Based Biosensor

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    ...Lars O., et al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality ...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ...would need much more guidance, direction, and a clearly laid out plan of action, others would act more ...

18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ... this question at my workshops, I get nods from nearly every participant. Robert Bramson, author “Coping...

19. Plasmids 101: Protein Expression

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    ... her PhD from Northeastern University and is particularly interested in cell signaling and communication...

20. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ... hits is then carefully reviewed. As mentioned earlier, it is common to find DNA from the packaging cell...