...difficult time. One function that rose to the challenge was our Finance Team. With Addgene closed for ...financial crisis. As a nonprofit organization, Addgene charges enough for our services to run our operations. ...to this need, we're incredibly grateful that the Chan Zuckerberg Initiative was able to step in to be ...laboratories all around the world." We thank the Chan Zuckerberg Initiative for supporting Addgene by ...

...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...secondary antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Antibodies 101: Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene....

.... CRISPR 101: Homology Directed Repair Addgenie Chari Cortez explains some of the basic science behind...contributors David Wyatt and Dale Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind...Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve...for your next experiment? In this post, Addgenie Chari Cortez guides us through some common controls and...

... wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration initiated at...Stanley Falkow, Stanley Cohen, Herbert Boyer, and Charles Brinton concocted a wild idea of using the newly...highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags...Plasmids that Glow" and "Viral Expression Vectors" chapters, and more pictures and pro tips.  Happy reading...

...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence..., and thus quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... cell heterogeneity, and/or cell position changes (To et al., 2016, Zhang et al., 2019). Thus to effectively...

...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem...could be played when and, depending upon who your challenger was, you might have to drastically alter your...please freely share your projects with us! This exchange of ideas will help improve life science and biomedical...

...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...attach important references that serve to further characterize a protein of interest (and we very much appreciate...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ... plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

...flexible work schedule the more we realize how casual chatter is very much missed in the virtual world. I believe...audience into small sessions and do a quick break to chat. This can be done randomly or manually assigning...could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social no-science-y...presentation will appear here, and only general chatter will be allowed with a different host each time...

..., the books center around a cast of endearing characters who decide to take matters into their own hands...the 1980s, Sarum follows an intriguing cast of characters from five different families over several millennia...one. On a purely selfish level, I wanted to be challenged to read some books outside of my comfort zone...s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate degree...

...Skip to Chapter 3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

...overall positive charge on DNA molecules so that it can more easily cross the negatively charged cell membrane...role in bacterial evolution and is the primary mechanism by which bacteria have gained antibiotic resistance...electroporation, the application of an electrical charge to cells, to increase cell membrane permeability...

... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment...biochemical processes. One of the largest and best characterized of these is the Src homology 3 (SH3) domain ...

..., but they do include HEPN nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded...Ruminococcus sp. (RspCas13d), while Konermann et al. characterized orthologs from “Anaerobic digester metagenome...Cas13a and Cas13b effectors. Like previously characterized Cas13 enzymes, CasRx also displays very high...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown...

...Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary projects...projects and collaborations. Summarized by Professor Michael Karin from UCSD: “The problem with cancer research...poster sessions During the poster session I got a chance to talk with Dr. Friedmann-Morvinski about tools...27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in cancer prevention...

...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate the effects...transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry ...hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of transduction...

...available in your machine before you make your purchase. Picking a single fluorophore for an experiment...overlap, or the signal "spilling over" into another channel's detection range. Though you can compensate for... University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core. Retrieved January ...flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf Syracuse Flow Core. Syracuse flow core spectral...

...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses ...cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: 10.1002/0471142735.im1017cs31...

...our mission, it’s also because Addgenies love any chance to nerd out over their favorite science topics....Addgenie expertise we can. We even have a Slack channel just for asking people if they can write or review...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...

...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...better than current technology and get customers to change behavior. When you are ready to commit to the company...