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TypeBlog Post...Proteins on the other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb...
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Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post.... J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...new family of seven ChRs derived from marine microorganisms based on metagenomic data. MerMAIDs (Metagenomically... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...recently deposited PSAM AAV, which encode engineered ligand-gated ion channels that can be activated with FDA-approved... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...important for nearly every aspect of life.” Unger began with the a bacterial periplasmic binding protein... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...of mitochondrial disease;” The Nuffield Council began their research into both novel and previously established... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...identification of research reagents, several journals or organizations now recommend or require authors to use specific... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...on stem cells and Alzheimer's Disease; and Kevin Eggan presented work that is leading to promising clinical... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...suggests new strategies to manipulate that process. We began our research with a simple question: how do Cas9... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...about reagents, and why other publishers and organizations have various different versions of checklists... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...they do and what they might be able to do and to organize it and catalogue it, I mean that’s the critical... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...no single fluorescent protein optimal for all organisms and that if you want the brightest signal, you... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...Bench setup Small adjustments in the physical organization of your workspace and your workflow can have... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...basics. Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...