We narrowed to 538 results for: invs

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   ... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

2. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...

3. A Guide to Starting Your Own Journal Club

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   Blog Post

   ... wanted to learn more, even those not directly involved in the project.  Once we gauged interest from ...

4. AAVs in Retinal Gene Therapy

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   Blog Post

   ...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

5. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ... for working with lots of students, managing inventories in the lab, or organizing a research project,...

6. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...interactive. If you can think of a good analogy that involves student participation, make it happen! How should...

7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   Blog Post

   ...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

11. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ...

12. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,...

13. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse...

14. Transferable Skills Guide: Creativity

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    Blog Post

    ...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

15. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP...

16. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ... Image from Li et al., 2021.   The updates involved the following changes. In pCas9, we 1) replaced...

17. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post

    ...reviewing this post! Dr. Ben-Simon is an assistant investigator at the Allen Institute in the department of ...

18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...of the developers of the MoClo system, are an invaluable resource for learning about MoClo in an in-depth...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...This process builds on our former approach and involves reading primary literature, soliciting information...

20. Hot Plasmids Spring 2024

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    Blog Post

    ...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...