We narrowed to 534 results for: invs
-
TypeBlog Post...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...
-
AAVs in Retinal Gene Therapy
TypeBlog Post...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... that even though these early steps don’t even involve antibodies, they are still critical to the outcome... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... for working with lots of students, managing inventories in the lab, or organizing a research project,... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... wanted to learn more, even those not directly involved in the project. Once we gauged interest from ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself. Caveats ... -
Hot Plasmids - August 2020
TypeBlog Post... at small and large scale. This new technique involves selection in the cytosol (instead of on the yeast... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse... -
Transferable Skills Guide: Creativity
TypeBlog Post...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...of the developers of the MoClo system, are an invaluable resource for learning about MoClo in an in-depth... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...