CRISPR 101: Ribonucleoprotein (RNP) Delivery
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Blog Post
...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...
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AAVs in Retinal Gene Therapy
TypeBlog Post...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... that even though these early steps don’t even involve antibodies, they are still critical to the outcome... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... for working with lots of students, managing inventories in the lab, or organizing a research project,... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... wanted to learn more, even those not directly involved in the project. Once we gauged interest from ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself. Caveats ... -
Hot Plasmids - August 2020
TypeBlog Post... at small and large scale. This new technique involves selection in the cytosol (instead of on the yeast... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...This process builds on our former approach and involves reading primary literature, soliciting information... -
Hot Plasmids Spring 2024
TypeBlog Post...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse... -
Transferable Skills Guide: Creativity
TypeBlog Post...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...