We narrowed to 534 results for: invs

1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

2. AAVs in Retinal Gene Therapy

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   Blog Post

   ...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ... that even though these early steps don’t even involve antibodies, they are still critical to the outcome...

4. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ... for working with lots of students, managing inventories in the lab, or organizing a research project,...

5. A Guide to Starting Your Own Journal Club

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   Blog Post

   ... wanted to learn more, even those not directly involved in the project.  Once we gauged interest from ...

6. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...

7. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

8. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ...

9. Negotiating Work and Life: How to Find the Joy

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   ...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,...

10. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

11. To Codon Optimize or Not: That is the Question

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    Blog Post

    ...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

12. Hot Plasmids - August 2020

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    ... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

13. Plasmids 101: Plasmid Incompatibility

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    ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...

14. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

15. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

16. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse...

17. Transferable Skills Guide: Creativity

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    Blog Post

    ...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

18. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...of the developers of the MoClo system, are an invaluable resource for learning about MoClo in an in-depth...

20. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...