We narrowed to 538 results for: invs

... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...

... wanted to learn more, even those not directly involved in the project.  Once we gauged interest from ...

...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

... for working with lots of students, managing inventories in the lab, or organizing a research project,...

...interactive. If you can think of a good analogy that involves student participation, make it happen! How should...

...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...

...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ...

...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,...

...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse...

...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP...

... Image from Li et al., 2021.   The updates involved the following changes. In pCas9, we 1) replaced...

...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...