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We narrowed to 850 results for: met

Showing: 461 - 480 of 850 results

  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies...including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used either with or...

  2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or...techniques. Thus, these proteins provide a versatile method to identify the expression of transgenes of interest...Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...pharmacological or genetic approaches, but only a few such methods exist and they only work for a subset of kinases...kinases. The Problem:  There is no common method for creating optically controlled kinases. The Solution:...KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating other signaling pathways...

  5. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...efficiency was similar to that of a comparable FISH method. Importantly, they did not observe labeling when...with both intronic and exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene...genome editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...panicked silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in...options were to stop talking until I could present something coherent or to quickly string together all the... above to make a strategy that can help. Even something as simple as telling yourself, “I can stop and...

  7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...been “working on new experimental and theoretical methods to push the scale of genetic engineering, with ...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...coaching plans that are financially accessible. Sometimes only a few hours of coaching time can make an ...Coaching. One nice thing about a group is that sometimes this offers the option of matching with the best...a good match as a coach for many scientists.  Sometimes it can be helpful having a coach that can offer...

  9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...there are for off-target edits to occur. Although methods to switch on Cas activity using light or drugs ... Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi....natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ..., author names and affiliations, introduction, methods, results, conclusions, and acknowledgements sections...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are...The other poster sections like the introduction, methods, and figures flank the central region, and a scannable...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...combination of these methods provides unique opportunities to develop computational methods to better understand...UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. PubMed PMID: 27992408. PubMed...

  12. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs. It consists...required if rAdV is to be used in vivo. The standard method for purification of rAdV uses cesium chloride (...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

  13. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... this blog post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols...Andrea Pauli and Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...minute! Fun fact! Electroporation is one popular method scientists use to promote transformation, but it...offer resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive ...inducible expression systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...relies on Gibson and other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    ...or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing...-relevant lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved...fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...controls, safety systems that are built into how something functions, were designed for this purpose. One...understand what safety precautions to take, and if something does happen, ensure you can receive appropriate... before you start an experiment, just in case something does happen.   Finally, the best way to be safe...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...concentrating a viral stock. Different titering methods can reveal differences in physical versus functional...the result of successful viral transduction. This method may not work if the virus does not have the proper... tropism to infect the packaging cells. Another method of ensuring viral production is to perform PCR ...

  19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...remarkable 12 working days. While the lab used this method for COVID-19 research, this protocol holds promise...System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148....

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012...Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org...
Showing: 461 - 480 of 850 results