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We narrowed to 779 results for: mos

Showing: 461 - 480 of 779 results

  1. Design Tips for Prime Editing

    Type
    Blog Post
    ...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    ...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    ...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...depending on what cell type it is expressed in. The most common target for chemogenetic manipulation are ...have been derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived ...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...

  14. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...

  15. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions — there are antibodies to ...
Showing: 461 - 480 of 779 results