We narrowed to 538 results for: sequence

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   ... use your fluoresent protein fused to a signal sequence that traffics the fluorescent protein to your ...

2. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...composition of the viral vector and the repeat sequences present in the LTR, the vector may undergo genomic...

3. Hot Plasmids and Viral Preps - May 2021

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   ...(A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape...

4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   ...currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control...

5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...pool (usually 10-30) of sybody candidates are sequenced for identification. All unique sybodies can be...

6. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   Blog Post

   ... combination with a synthetic library of TadA sequences to create the eighth generation of adenine base...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ... color and application Almost as soon as its sequence was elucidated, scientists began engineering new...

8. University of Florida Serotype Testing Panel for the Eye and Brain

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   Collection

   ...and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. These ...

9. Viral Vectors 101: The Retroviral Lifecycle

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   Blog Post

   ...ssRNA strands (which can have a different genomic sequence from one another) — by various mechanisms, only...PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging two RNA genomes in one retroviral...

10. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...developed methods for selecting and amplifying RNA sequences that could bind very specifically to target molecules...

11. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post

    ...also arise from random mutations in the genetic sequence that happen to be beneficial, and persist as a...

12. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post

    ... can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody is not ...

13. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ...virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods, 23(1), Article 1....

14. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed...

15. Lentiviral Vector Uses and Overview

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    Blog Post

    ...with a lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients...

16. The Challenges of Cell Culture

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    Blog Post

    ...testing, from animal doping to forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn...

17. Plasmids 101: Protein tags

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    Blog Post

    ... An affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash...

18. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ... and found upstream of their target genes. The sequence of the promoter region controls the binding of...

19. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts and stop codons...

20. Hot Plasmids: Fall 2024

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    Blog Post

    ... (Cao et al., 2024). The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-...