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We narrowed to 1,052 results for: sta

Showing: 461 - 480 of 1052 results

  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...AAV.  Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you...endonuclease domain. Following replication, Rep78/68 stays attached to the AAV genome and docks with the genome...Alternative splicing and differing translational start sites generate the three VP proteins. The three ...clearly defined yet. Now you’ve got a basic understanding of the AAV RepCap plasmid! Check out this blog.../doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava A (2019) Capsid Modifications for Targeting and...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    ... of the project. The state of research is constantly shifting, and long-distance collaborations are becoming...between each meeting, but short enough that everyone stays on the same page. Ideally, these meetings include...Conference in Frankfurt, Germany When funding and circumstances permit, think about having collaborators spend...communication within an existing collaboration, or start something new! Additional resources on the Addgene...

  3. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...new to plasmids may need some extra guidance to understand the specific components that make up a plasmid...and techniques, and empower you with a firm understanding of the fundamentals. Our mission is to curate... more functional benefits to the host such as resistance to antibiotics, degradative functions, and/or...advantageous for survival, such as an antibiotic resistance gene. In contrast, plasmids utilized in the ...recruiting replication machinery proteins Antibiotic Resistance Gene Allows for selection of plasmid-containing... obtained. Selectable Marker The antibiotic resistance gene allows for selection in bacteria. However...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...was using.  I started to do little things to save reagents and money.  This was the start of my interest...resources where possible.  At the lunch table, for instance, we have a plastic container problem…reusable ... a popular topic in the media.  At one point I started to become very aware of how little care I took ...time and money. My other stingy science project started when I realized I was pouring large agarose gels... a small portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Addgene Blog? Fill out our guest blogger form to get started!   Thanks for all your hard work!   Fluorophores... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...important things to consider if you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR ...efficient genome editing - all influenced by an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking...

  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...choice while bypassing the intermediate pluripotent state. Though a valuable tool for regenerative medicine...These library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build...reprogramming treatment (CellTagD3), and 13 days after the start of reprogramming (CellTagD13), which is when the...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In this lineage...putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail...

  7. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...shipping staff agreed to come in and start getting plasmid deposits into the collection and to start shipping...closure was earlier than most companies. Since the start of this crisis, we’ve been very responsive to the...Addgenies home.  At the same time, as Addgene started dealing with this new reality of working remotely...processes are entirely self-supporting. We hire more staff as we need them to meet demand and to serve the ...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. .... You just need three plasmids to start making AAV:  the packaging plasmid which contains the structural...between the ITRs is ~4.4 kb. ITRs are inherently unstable due to their secondary structure, palindromic ...palindromic nature, and high GC content. This instability can cause partial loss of an ITR during routine plasmid... Plasmid video!   Now you’ve got a basic understanding of some common AAV plasmid elements! And while...

  9. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...towards a career in science communication, an established field of inquiry with which I was not yet familiar...Herein, a good science communicator—with an understanding of how to appeal to a given audience and how... a scientific background and bring with them established interests and areas of expertise. Perhaps the...individuals respond to issues of unsettled science. Understanding just how the public utilizes insights gleaned... ours reflect a growing effort to address this obstacle in its many forms. A short stroll from my office...by the day, it has never been more crucial to understand just how we receive, process and respond to that...

  10. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...Tchetchelnitski V, Mehmet H (2012) Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia...culture, only the plasmid with the antibiotic resistance gene and origin will replicate, and the transgene...recombined vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and...Recombinase-deficient strains like Stbl2, Stbl3, and NEB stable E. coli reduce the rate of recombination in samples...CS, Esposito D (2007) Improved recombinational stability of lentiviral expression vectors using reduced-genomeEscherichia...

  11. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... use since the 1980s. “Synthetic dyes were the standard in the field, but there is one problem: how to...biochemistry of living neurons in an effort to understand the connection between molecular- and cellular-level...elegans, and zebrafish – essentially all of the standard organisms for which extensive genetic tools are...presence of a ligand (they stick together in one state, but not the other). “It’s quite a robust way to.... “I hope by depositing at Addgene, people will start using them,” Merkx said. “For us, it is one of the...developed something useful [enough] that other people start using it. For us, it’s like a test.” References...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...use grows across the life sciences and to establish standardized validation techniques for academia, industry...both Surveyor™ and Sanger may not be adequate standards for validating edits.  Mismatch cleavage assays...Tsiatis et al. 2010). As the field moves toward standardized thresholds for validating CRISPR experiments...breaks Prediction algorithms: A good place to start for biased validation At the moment, many software...sequences of the genome and sgRNA. This is a good starting point for most researchers as it provides a list...approval. It is key to maintain a consistent set of standards as the field seeks to generate reproducible, quality...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...of this workshop? What do I understand already? What don’t I understand? How does the information I’...prevents it from forming concatemers later. The "FS" stands for Fragmentation System and this is the most important...libraries from small amounts of DNA i.e 100 pg. At the start of the workshop, NEB gave a brief presentation describing...hoped, we found that regardless of how much DNA we started the process with, we got great amplification of...is extremely important for us as a company to constantly update our processes and get new ideas from learning...

  14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...cells treated with MG-2I dye and exposed to light stained dead within 30 minutes of exposure. 90 seconds ...killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments with ... 2). Induced cell death was confirmed by TUNEL staining.  The video below shows what happens to the zebrafish...as Mg-2I-dL5**. Mg-2I-dL5** also had higher photostability than KillerRed, with KillerRed reaching greater...ablation while having limited collateral damage to bystander cells. Are you ready to use MG-2I-dL5** with your... D. M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V., . . . Lukyanov, K....

  15. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...You’ve prepped your DNA and you’re ready to get started on the next step of your experiment. But in many...through a liquid to determine the concentration of substances in the liquid. Molecules absorb different wavelengths... methods, fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity... a fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating...microarray studies and is not used as much for the standard plasmid prep. Diphenylamine method Another absorbance...made in the visible range and can be read with a standard ELISA reader if no other instruments are available...

  16. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...says Bachle. (For presenters, there’s also less stage fright!)And depending on the conference, the audience... questions,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question... the app or virtual platform and making sure you stay connected. If possible, test out the technology ...conference begins.“There were some concerns of having a stable internet connection,” says Abitua about her workshop...chats between sessions or waiting for a session to start. “There was not much time to network,” Zhang says...discussing at tables and the event hasn't even started yet! @neurosymp will be interesting this year. ...

  17. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...fascinated by the human body and how it works. I used to stay up past my bedtime, poring over my grandparents’...science made me happy, but it wasn’t long before I started becoming disillusioned with the gulf between what... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...in neighbouring disciplines might struggle to understand. Why should we trust something that we cannot...know and believe about the world. It is vital to understand what people know – or, at the very least, believe... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

  18. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...suppression (and most everything in between), understanding how, when, and where TFs work is of broad interest... 3,500 human TF isoforms, to help researchers understand this regulatory network. The transcription atlas...fates. The atlas of directed differentiation To understand how each human TF can contribute to differentiation...endeavor, a map of possible TF-induced cellular states was generated (Joung et al., 2023). Fig....screening approach can not only lead to a better understanding of disease onset, but also better simulate a...is a valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte...

  19. The MTA at Addgene

    Type
    Blog Post
    ...the community to understand and grow the scientific ecosystem, including understanding how shared materials...  An MTA is simply a contract or agreement that states the terms of use for a tangible research material...process, which eventually led to the creation of a standardized agreement on the transfer of biological materials...with many properties, having well-understood standardized terms makes it a lot easier for organizations...One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which your material will be distributed...

  20. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...worked with many Addgenies to help improve the understanding, curation, and explanation of our CRISPR resources....” Doench’s graduate work helped expand our understanding of how miRNAs regulate their targets and what...screens to answer their own research questions. Starting at the Broad Institute For his post-doc, Doench... technology development alongside it.” Doench starting working with David Root in the then RNAi Platform...left him a bit skeptical of the technology at the start of the CRISPR craze, but he says, “In the case of...Involvement with Addgene Although Doench’s thorough understanding of CRISPR is what got him involved with Addgene...
Showing: 461 - 480 of 1052 results