.... Alternatively, you can perform simultaneous detection with a mixture of primary antibodies and appropriate..., M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex...Introduction to Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence...

...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

...repair of the break to allow for site-specific detection downstream. The con to these approaches is that...that NHEJ must perform the repair to allow for detection, not Homologous Recombination or Alternative End...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

... Why might one be interested in state machine technology? Biological research has produced a massive amount...itself to cheap, quick, and high throughput state detection with flow cytometry. However, expressing reporters...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

...developing an easy to use diagnostic tool for the detection and diagnosis of different types of pancreatic...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

... good correlation between the levels of target detected in both methods. These approaches have relatively...are ultimately planning to use your antibody to detect endogenous levels of the target in other cells ...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...

.... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

...even if they are present in the sample. Other techniques, like electron microscopy and FRET, can offer...details are still being uncovered, and different techniques all bring their own advantages—and blind spots—to...RNA-programmed Cas9 nuclease specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt...

... looking for the best way to improve your lab techniques — but perhaps are a little limited on time — ...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...

... some best practices for introductory science techniques. It’s also great for teachers looking to supplement...their curriculum. From the details about personal protective equipment to learning about how to use a tissue...

...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ... at MIT. He also writes Codon, a blog about biotechnology.    ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...

...recent advances in the biological sciences and techniques that simplify and expedite research. We recognize...through our blog. Do you have skill in a particular technique? Do you have a set of tips you give to every new...

...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

... with our 101 series, which introduces common techniques and concepts. But what if you’re looking for ...Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head...

...ways to use CRISPR in plants, to learn techniques for detecting genome edits, to discover why you might...

...is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to... to law school. This was in the early 80s, and biotech was really just taking off. I found out that patent...

...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...

...repositories provide standardized quality-control and technical support to the community. Thanks to Our Guest ...Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: ...