We narrowed to 1,073 results for: TOR;

..., Word docs, and the like is likely stored in online file storage tools like Dropbox, Box, One Drive, ...commonly used online file storage tools, as illustrated in this video, and to data stored on institutional and...source data repositories Over the past decade or so a number of general purpose repositories have been ... in the repository after the deposit has been made. It’s possible to associate the depositor’s ORCID ID...value the repository integrations, because when RSpace documents are deposited into the repositories, links...research; and (3) connectivity to open source data repositories. To illustrate this, let’s take a look at the...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the...

...of the most compact genome editors to date. We have developed multiple vector versions for TnpB-mediated...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant...the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested vector using your ...Precise plant genome editing using base editors and prime editors. Nature Plants, 7(9), 1166–1187. https...blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute...efficient delivery into cells, particularly via viral vectors. While viral delivery is a highly effective method...

...HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors that utilize exosomal... to track epidermal growth factor receptor (EGFR), a transmembrane receptor tyrosine kinase essential ...EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors present unique ...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization Work conducted...This method offered kinetic, non-invasive monitoring of receptor dynamics at the cell surface. Find HiBiT-EGFR...interactions with co-receptors (like NRP1) and by endocytic trafficking of the receptor. Peach et al. (2021...to subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods...been used for tracing and visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...and Mooney, R. (2017). Identification of a motor to auditory pathway important for vocal learning. Nat ...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily... low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high ... is often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre...

... edit (or edits) engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to...range of specialized prime editors (PEs). PE6a and PE6b are small prime editors with RT domains derived ...genome editing methods using nucleases and base editors have the potential to correct only a minority of...the edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia.... We’ll summarize the major ones in Addgene’s repository later in the post. Advantages of prime editing... constrained by PAM sequence location The prime editor extends the reach of CRISPR genome editing, as ...either DNA strand, many previously developed base editors with a <8 base-pair editing window cannot edit ...

... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...additional information or clarification on these vectors. Read on to find the answers to our most frequently...questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage...years based on HIV-1; however, the 1st-generation vectors are never used as they pose too much of a biosafety...

...large size of Cas9 activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by ...mCherry (vector 1) with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed...Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester Medical Center...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites...

... viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...of an Addgene AAV viral vector preparation after negative staining. Empty vector particles can be identified...darker than full vector particles. This image shows that the vast majority of the vectors consist of full...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...which is a factor in AAV titration since the starting template is the purified viral vector solution (...feasible qPCR strategy for titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed...many ways to quantify the composition of a viral vector solution, and generally these methods measure different...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...