...into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate, for Stage 3, that the...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages Learn...

... dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary environment...Utilize your office space too Balance point number 3 with doing fun things within your office. This way...early employees spent this remaining time by elaborately pranking each other, dressing in costume, and...frequently ask the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all day long, whistling...megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring...

...the organization(s) you serve time and resources. 3. Get a mentor, find your people Find someone who is...GPCI evaluates a candidate's expertise (a minimum of 3 years experience) and experience through the candidate...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...

... for an extended period of time (>10 passages or >3 months). Other times cell lines are unavailable from...State University of New York Press, Albany (1986). 3. Masters, John R. "HeLa cells 50 years on: the good...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...interfere with it in a BSL-2 lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents... CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 ...different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see other plasmids... optimized. Browse the collection or learn more about it on the blog. COVID-19 research is happening fast...through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository...Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction... interaction Find tips for remote collaborations Browse all COVID-19 blog posts Resources on Addgene....

...enhancers 3. CRISPR out the enhancers from step 2. See which ESCs die or differentiate Step 3 reveals which...republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt...tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We’ll start ...discovery and therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section...Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine...on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit...

...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus...SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9...jejuni (CJ) 3' NNNNRYAC Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW...characterized *In the table above, 3' and 5' indicate on which end of targeted sequence the PAM is located... DNA target, the seed sequence (8–10 bases at the 3′ end of the gRNA targeting sequence) will begin to...gRNA will continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches...matters. Mismatches between the target sequence in the 3′ seed sequence inhibit target cleavage, while mismatches...

... Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John ...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

...type and the specific genes cloned into the vector.3  2A Peptides To overcome some of the disadvantages...et al. (J Virol. 1988 Aug;62(8):2636-43.) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith...genes above with your gene or genes of interest. Some of the plasmids listed in the table above (and their... an internal region of the mRNA. In the figure above, you can see that the IRES element acts as another... first discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of ...

...Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR...pathway (Charpentier et al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small... post on increasing HDR efficiency to read more about optimizing this distance. Homology arms and types...screening strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help...