We narrowed to 641 results for: ato

...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...trials for other diseases, including the common hematopoietic disorders sickle cell anemia and beta-thalassemia...

...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

...and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing appropriate...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...

...anxiety by modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal...shRNA to downregulate expression of miR124a, a regulatory RNA that negatively regulates BDNF levels. They...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

...splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity and does not work...phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully implemented...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...& Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have suggested ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs... https://doi.org/10.1038/nature14299  Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, ...

...within the enhancer sequence, then interact with mediator complexes and the RNA polymerase at the promoter...reviewing this post! Dr. Ben-Simon is an assistant investigator at the Allen Institute in the department of ... . . Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron, 109...

...but not all. For example, LDLR expression in hematopoietic stem cells, T cells, and B cells is low. Infection...great at infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses...