Viral Vectors 101: Producing Your rAAV
Type
Blog Post
...reagents and keep the cells happy.
Harvesting virus
Once you have transfected your cells, wait 2–5 days and...however, you’ve collected the producer cells, you’ll need to lyse the cells to let the virions out. We recommend...particles that can transduce cells, which can vary based on target cells and can be altered by freeze/...come into contact with the rAAV and apply common cell culture practices diligently throughout the production...capsid genes.
In this helper-free system, HEK293T cells, expressing Ad E1A and E1B, can be transfected with...and then collect the supernatant and/or producer cells for further processing. What component(s) you need...released into the culture media, while others remain cell-associated (Vandenberghe et al., 2010). For certain...