We narrowed to 643 results for: ATO

1. To Each HIS Own

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   Blog Post

   ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...

2. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...

3. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...and were presented at the ASPB meeting by the collaborators who could participate. Despite the group not...

4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet all of them. These...

5. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   ...storytelling is an impactful tool for science communicators. “The power of a story is to make a human connection...

6. RNA Extraction Without A Kit

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   Blog Post

   ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

7. Transferable Skills Guide: Cross-team Communication

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   Blog Post

   ... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

8. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

9. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...

10. dTAG - You're it!

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    Blog Post

    ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...

12. Summer SciComm: Preprints

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    Blog Post

    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...

13. Summer SciComm Series: Cognitive Load

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    Blog Post

    ...help:  If there’s a teacher, mentor, or other educator working with your audience, ask! They are experts...

14. Antibodies 101: Affinity Reagents

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    Blog Post

    ...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...

15. Human Germline Editing Using CRISPR

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    Blog Post

    ...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

16. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates...

17. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...

18. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct order in...

19. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ...

20. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...