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  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...for scientists to understand how GPCR activation leads to downstream effects.  Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and an Addgenie ...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the Cas9 nickase which will together introduce cleavage of the target site with efficiency similar to ...system, including one recently from the Zhang group. Learn more here. Q2: When designing oligos for cloning...

  8. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your...

  10. Meet the New Addgene Board Members!

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    Blog Post
    ...Association and it was through this connection that she learned more about Addgene. “The mission to share critical...and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has...

  11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux...

  12. RUBY-Red Siliques

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    Blog Post
    ...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ...

  15. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur...

  16. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...

  20. Addgene Depositors Get More Citations

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    Blog Post
    ... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe...

  21. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city...

  22. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ...

  23. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR...

  24. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical...

  25. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

  26. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ...

  27. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  28. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  29. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  30. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...
Showing: 721 - 750 of 1014 results