We narrowed to 1,104 results for: Ide

... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost...

...sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links...

...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...

...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ...

...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition...

...make them decide to read (or listen or watch) further.  Second, the introduction needs to provide enough ...

...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

... schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible...

...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours and...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ...

...Professor Max Krummel from UCSF showed some impressive videos of these interactions (Ref 3). You can find useful...tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the conference aspirin and the use of...

...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...incorporating chemically inducible approaches to provide temporal control, this new system, Fkbp/Frb Inducible...

...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

...synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

...the respective hairpin binding domain. Pairs of identical hairpin sequences specify the primary colors blue... colors and a white composite. gRNAs with two identical hairpin binding domains produce blue, green, or...

...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein ... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

... excited about. Following scientists in fields outside your main research focus lets you keep a pulse ...window of time for publication. To help you quickly identify papers with greater influence, the average number...

... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...

...leadership and management skills. The NPA brought outside experts and many hands-on- and role-playing exercises...with other leaders in my research field should I decide to remain here.  In a time when it is so important...

...stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence. Users can design gRNAs to induce...SapTrap, scientists will be able to generate genome-wide libraries containing fluorescently-tagged proteins...

...strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive listing of...often ambiguous and should be viewed more as a guideline, since they are typically based on the copy number...

...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

...expression. Take a look at all of the options and decide what might work best for your experiments! This...Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA...

...orange and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns...bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports 7, 42398. PubMed PMID...

...channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...

...working with viral vectors, but what we lacked were videos guiding completely new members of the lab or those...

...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave...

...decision between some meaningful names…. Watch the video to find out the final four! (Note: For cases where...

... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

...editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene gave to...

...in the scientific community. Addgene has been considering a gradual move off X as a response to major platform...