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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 481 - 500 of 838 results

  1. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...are distributed. Next month, we are making two changes to the policy that will make the process a bit ...updating our Hold for Publication policy with two changes that will help us ensure that plasmids don’t get...

  2. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid pages We’ve given...RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue for temporary...

  6. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...29853555. PubMed Central PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor...

  7. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental...

  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural...

  10. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...contamination.  Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...for the sugar backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020).   I ...  References and Resources References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively...

  17. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...

  19. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...
Showing: 481 - 500 of 838 results