... an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The...

..., etc). The cloning method is ultimately chosen based on the plasmid you want to clone into. Regardless...

...lab and into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others...

...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...

... cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI...

... genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors...

...audience can understand the conflict. A science-based conflict gives you an easy framework with which ...

...Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains...

... search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,” “associate...

...newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required...

...will receive much better health care in the future based on our studies.” Thank you to all the BeHeard Awardees...

...Douglas K. Smith or the book on which this article is based.          ...

...Fly vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into...

...synthetic compounds. Antibiotics can be categorized based on whether they directly kill bacteria (bactericidal...

...against unnecessary clinic regulations, evidence-based research is vital in combating misinformation and...83.7430°W – Alexandra Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan...

... a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007)....opportunities, it’s an exciting time to be in the lab. Based on the power of available techniques and how quick...nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to detect a BRAF ...strand from the control cells, which should contain basepair mismatches at the site of the edit. Using Surveyor...

...generally high in As and Ts. Why, you ask? Well, A-T base pairs are held together with two hydrogen bonds ...

...calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are not suited ...

...expected something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to...

...be distributed for research use only to industry-based scientists. Some organizations choose to share their...