We narrowed to 717 results for: CaS;

...astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G that generally mediates broad cell tropism... or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be ...

...large (1–10) and small (11) fragments. In several cases an FP(1–10) fragment may be compatible with multiple...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

...conjugated substrate is added to the wells. In the case where the detection antibody is biotin-conjugated... protein within a mixed sample. However, in this case your protein of interest is pre-incubated with a...

...developed nucleic acid detections tools based on Cas12a and Cas13  which could be repurposed for SARS-CoV-2 ...

... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...

... that expresses well, then probably not. In this case, take a look at the secondary antibodies that your...to probe multiple targets in parallel. In these cases, it is critical that users choose antibodies that...

...the heading "Addgene Partial NGS Result." In some cases, there will be more information about our sequencing..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

... providing feedback is needed for that. In many cases reports are not aware of issues until you bring ...what to do. Unfortunately this is not always the case or you may not feel comfortable reaching out to ...

...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

...the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into its adult form...eclosion, the process of hatching from their pupal case (~9 days after setting up the genetic cross). You...

...potentially costly set-ups. However, there are many cases where the specific viral particles you need are ... viruses are too high for your budget. In those cases, you’ll need to produce your viruses in-house. ...

...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....