We narrowed to 753 results for: ELP

1. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...Thankfully, there are many great resources available to help guide you through the rough waters of mutation validation...

2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...research, modeling patient mutations using SDM can help determine if a variant is causal for a given disease...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...of virus with various tropisms.  Packaging using helper-free packaging cell lines This method utilizes ...

4. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   ... blogs on virus safety and viral applications to help you start (safely!) thinking about the possibilities...

5. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   ...look at an outcome. Your scientific question can help determine which is right for you. Screen variations...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   ... homologs. Evolving better base editors with the help of phages! The latest ABE from the Liu lab is ABE7.10...

7. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ...materials. The use of reporting guidelines can also help remind us to include all of the essential details...

8. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...involved). The new homepage and navigational elements help users who prefer to browse and discover items in...

9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...advantages and limitations of each Cas enzyme can help determine which enzyme to use. Remember, accessibility...

10. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    ...replication-incompetent phage particles with assistance from a ‘helper’ phage prior to transduction. Scientists also use...

11. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ... cell type is already known, these reporters can help define the different cells present in a mixed population...

12. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ...tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate the evolution...

13. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    ... viral vector service in 2016...we’re excited to help scientists share their reagents with the scientific...

14. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    ... and disadvantages of monoclonal antibodies will help you make the best decision for your experimental...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...efficient, what other challenges does this system help to solve? Myers: Only a small percentage of the ...

16. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    ...brom-indigo). So far, so good, but how does this help us screen plasmids? Blue-white screening in the ...

17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ...P65-HSF1 plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide...

18. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ...  Yeast selection marker What does the marker help synthesize? Counterselection? (growth-based positive...

19. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially in the...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...