We narrowed to 802 results for: Eng

1. Management for Scientists: Managing vs. Leading

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   Blog Post

   ...motivating their doers by ensuring they are as engaged as possible. To learn more about being an effective... and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders...

2. Management for Scientists: What Makes a Good Manager Anyway?

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   ... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered...

4. RUBY-Red Siliques

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   ... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Additional resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

5. Hot Plasmids and Viral Preps - September 2021

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   ...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...

6. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...

7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...23817127. PubMed Central PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index...

8. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to...

9. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

10. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    ...are single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels proximal proteins...

11. Identifying Sequence Elements with SnapGene's Feature Database

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    ...controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions...

12. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post

    ...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...

13. Science Careers: Unruly Interests Feed Many Paths

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    ...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

14. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100...

15. Addgene's Top 10 Blog Posts of 2015

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    ...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...

16. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...different wavelengths of light to different degrees and many molecules have a specific wavelength that they...

17. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...distance.  From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been...

18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... by directed evolution. They then optimized the length of both linkers to generate a reporter that would...

19. Addgene Depositors Get More Citations

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    ...from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

20. With Our New Viral Service, We're Taking CRISPR Further!

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    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...