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We narrowed to 1,145 results for: Gene

Showing: 481 - 500 of 1145 results

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    Type
    Collection
    ...Genomics Consortium Plasmids All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry...

  2. The SGC Toronto Collection

    Type
    Collection
    ...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post
    ...sites yourself using PCR, but gene synthesis can save you time. Using gene synthesis, you can request the...If you’re creating a multigene construct, in what position do you want your gene to be in relation to ...to the rest of the genes? And do you want it to be in the same (forward) or in the opposite (reverse) orientation...you a lot of headaches down the line! Cash in on gene synthesis Before you start your cloning, it is advisable... Figure 1: General overview of the MoClo assembly process to produce a multigene construct. Figure...Golden Gate Cloning Additional resources on addgene.org Addgene's MoClo Guide and Kits MoClo Toolkit MoClo...chosen one of the MoClo kits available through Addgene that suits your experimental purposes. But what...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...tags for live imaging in C. elegans CRISPR/Cas9 for gene disruption in M. abscessus  Bioluminescent reporter...developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly...Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or stepwise...system is induced to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ... Sean C., Genevieve Laperriere, and Hugo Germain. "Droplet Digital PCR versus qPCR for gene expression...vectors and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. PubMed...genomes can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson...virus type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID: 20486768...benchmark for infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879. PubMed PMID: 20336156...new viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

  6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post
    ... as ‘indels’. While this is convenient for generating gene knockouts, NHEJ repair does not allow introduction...CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms and model systems. We ...were able to introduce the desired mutations in genes associated with dementia, such as APP and PSEN1,...Cas-blocking mutations at both targeted alleles when gene editing.   Figure 1: Techniques to improve...called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction of an intended...introduction of specific sequence changes. To generate a specific sequence change the cell must undergo...that desired genome-editing events by HDR can be generated more efficiently by (1) improving editing accuracy...

  7. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...scarless cloning. The cloning scheme is as follows: the gene of interest is designed with Type IIS sites (such... allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct...recognition site(s) thus eliminating these from your gene of interest. PCR products are then digested with...ligated following a heat inactivation step. If your genes of interest or destination vector contain multiple...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based gRNA assembly methods...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...presence of the protein endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains ...plasmid amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease...nuclease activity. For strains that still carry this gene, there are usually extra steps like heat inactivation...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit...Browse our Plasmids 101 blog posts Resources on Addgene.org Check out the Molecular Biology Reference Find...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...biosensor, by Addgene’s categorization) was first developed in 2001, with successive generations developed...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...viruses engineered to be safely used to deliver a genetic payload to cells, and the complexity of effectively...

  10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post
    ...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...

  11. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us ...sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality of working...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...rose to the challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide...

  12. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer’s needs...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... that we needed to improve more than that: the general “findability” of the information on the site. Different...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

  13. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post
    ...the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta... is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

  14. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ... chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping us make Plasmids...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ... purple bacteria video background with mascots Blugene and Aavery? Download the 720p version or download...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ... green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background...

  15. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

  16. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post
    ... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

  17. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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    Blog Post
    ...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

  18. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ... orientation as the gene or use custom gene synthesis or site directed mutagenesis to remove the BbvCI...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in the plasmid...Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate Specific Mutations in Your... pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries...of this. Plasmid library generation prior to one pot saturation mutagenesis Several methods can be used...effects. One-pot saturation mutagenesis The one-pot saturation mutagenesis technique described in Wrenbeck...

  20. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...Versatile Tool for Gene Expression in Mammals Additional Resources on Addgene.org Overview of Viral ...pooled libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform...while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV AAVs ...associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral...
Showing: 481 - 500 of 1145 results