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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 937 results for: KIN

Showing: 481 - 500 of 937 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...as well as high spatial and temporal resolution, making it ideal for visualizing gene expression in vivo...ultrasound contrast. We (the authors) are currently working on improving and optimizing ARGs, with Marjorie...They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background tissue...intraperitoneal doxycycline injections to enable their tracking with ultrasound imaging (Fig. 2d). Creating a ...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...situation differently, some adopting simple note-taking tools, others using excel sheets or organizing ... on the market today, ranging from general note taking tools, to more scientific field-based solutions...of use of the software. We identified that when talking about ELN costs and limited budgets, “a large percentage...ELN will support that. In addition, if you are working for a large institution or company, site licenses...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies that involve...genetics and developmental patterns are conserved, making them great models for human disease research. One...eggs are large, opaque, and produced in abundance, making them useful for in vivo studies. These attributes...flowers. Image from BlueRidgeKitties. If you are working with Arabidopsis check out the GreenGate Toolkit...

  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...fit a big gene into a tiny vector like AAV? By breaking the transgene into smaller pieces. Reassembly ...following transduction.  So what’s going on here? The working model is that the oversized AAV genomes are fragmented...Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261 . https... Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...experiment. What is immunofluorescence? Generally speaking, IF is an application in which antibodies that... different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers...different colors. Multiplex immunofluorescence Speaking of different colors, having a palette of secondary...permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific binding...

  6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...forever!  This can be an advantage for researchers looking to generate long-lasting changes in cells. However...inserting viral DNA into the host genome. Without a working integrase, IDLVs can still deliver their genetic...Considerations for IDLV use If you’re reading this and thinking “Gee, I’d love to give these a try myself,” look...Linch, D. C., Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von...

  7. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Brainbow labeling has also been applied to cell marking and lineage tracing in other systems, such as zebrafish...directly trafficked to cell membranes. Membrane trafficking of farnesylated derivatives enables labeling ...fluorescence due to the addition of translation-blocking STOP cassette immediately following the promoter...dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R,...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...this post, we didn’t come across a broadly used working definition of systemic capsids. After some consideration...Beadle, K., Jang, M.J., Kim, H.M., Rajendran, P.S., Tompkins, J.D., Shivkumar, K., Deverman, B.E., Gradinaru...Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E.... I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu, C., & Deverman, B. E. (2022). A high-efficiency...C., Lagerborg, K. A., Tellez, L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M.,... Tabebordbar, M., Lagerborg, K. A., Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli...

  9. DNA Quantification

    Type
    Protocol
    ...DNA is resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...certain Cas enzymes are less compatible with the bulkiness that accompanies mispairing of bases. To reduce...enzyme swaps will only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator...

  11. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... channel with fast kinetics may be required, whereas a channel with slower kinetics can be used for lower...each light source used.   Channel and activation kinetics Some channels turn on and off very rapidly in ...thorough characterization data, like spectral and kinetic activation information, in the supplemental data...

  12. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...counting the number of puncta in each cell. When making figures for a paper or presentation, make sure ...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...

  13. Protocol - How to Design Primers

    Type
    Protocol
    ...regular nucleotide for broader pairing capabilities. Taking into consideration the information above, primers...

  14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...stability. Optimizing antibodies for better pharmacokinetics and reduced immunogenicity allows for more...the specific mutations you choose, whether it's tweaking the antibody-antigen interface or fine-tuning ... mutants for the specific functionality you’re looking for. For example, if you were aiming to increase...-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across...

  15. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... single dependent variable. I know what you’re thinking – this really sounds like a two-way ANOVA. And...calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates... you want to know type of distribution you are working with, graphing data with a histogram is sometimes... friendly neighborhood biostatistician happy by making your statistical analysis plan when you design ...

  18. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...eBook collection is a handy resource for anyone looking to use antibody-based applications in their research...: What is an Antibody” quite useful. If you’re looking to design your first flow cytometry experiment,...the Antibody 101 eBook is a great place to start looking for answers.    We hope you find the latest addition...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying...pressure-test your FRET assay to make sure it is working? This article addresses these questions, which ...s best to consider these cases separately when talking about the tools that are available. Let’s consider...Denis V. Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." ...

  20. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...Fly-related Issues in the Lab A significant drawback of working with fruit flies is their ability to escape their...experienced the minor yet consistent annoyance of walking into a fly lab and finding ourselves accosted by...system in a video filmed at Addgene:     If you're working in fly research, I hope you find this new technology...
Showing: 481 - 500 of 937 results