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We narrowed to 783 results for: Mos;

Showing: 481 - 500 of 783 results
  1. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...
  2. Pooled Library Amplifications

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    Blog Post
    ... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...
  3. PhD Applications After COVID

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    Blog Post
    ...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...
  4. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...
  5. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...
  6. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...
  7. Tips for Using BLAST to Verify Plasmids

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    Blog Post
    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...
  8. Getting to Know Expansion Microscopy

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    Blog Post
    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...
  9. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post
    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...
  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...
  11. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post
    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...
  12. QC Sequencing Technologies at Addgene

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    Blog Post
    ...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...
  13. Hot Plasmids: Fall 2024

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    Blog Post
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...
Showing: 481 - 500 of 783 results