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We narrowed to 770 results for: Mos;

Showing: 481 - 500 of 770 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    ... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions – there are antibodies to ...

  3. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...

  4. PhD Applications After COVID

    Type
    Blog Post
    ...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...

  5. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

  12. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

  14. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

  16. Adenovirus Guide

    Type
    Guide
    ...is the most popular method for creating first-generation rAdV vectors, which is still the most commonly...range of mild diseases in humans and other animals, most commonly respiratory infections, such as the common...applications. However, this has also made them the most widely used viral vector platform for vaccine design...vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus type 5 (Ad5...autonomously. First-generation rAdV vectors remain the most popular choice due to their well-established methods...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially long transgenes, the use...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...
Showing: 481 - 500 of 770 results