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We narrowed to 761 results for: Rest

Showing: 481 - 500 of 761 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors to VP1,...into a compartment encapsulating the protein of interest (Figure 3).   Figure 3: Schematic representation...

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly...

  4. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ... spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel...pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Watch the Video!...new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short stretch... Cloning (LIC) Scarless cloning with Type II restriction enzymes and T4 polymerase pLKO.1 - TRC Cloning...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

  11. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...experiments with/for them. Though not the most prestigious role in a company, Technical Support is essential... open to experienced professionals with strong interest in the technical aspects of science and the drive...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...understandable to scientists outside your field, and interesting. For example, “there is a significant need for...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Let's Go! How to Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid...Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest Analysis We also introduced guides...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... answers to such questions, Addgene is deeply interested in pursuing them. We’ve recently revamped our...can help others as much as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...scientists can make mutations throughout a gene of interest based on an organisms codon usage bias to increase...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful...
Showing: 481 - 500 of 761 results