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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 65 results for: Rest

Showing: 1 - 20 of 65 results

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...Adult Dermal Fibroblasts Neurons Lentiviral Human REST suppression mediates neural conversion of adult ...

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a 140068 Anti-Kv1.6...GluN2C/NR2C Rat Mouse IgG2a 222145 Co-Rest/RCOR1 [K72/2R] Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin...

  4. Bacterial Expression Systems

    Type
    Collection
    ...protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction enzymes...relatively well understood biology to answer many interesting questions. Does my transcription factor bind ...and/or alter cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best...Plasmids Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can be transposed... many enterobacterial genomes. The bacteria of interest are transformed with the appropriate construct...get a better understanding of what your gene of interest does when expressed in vivo . Plasmid ID Visualization...fluorescent protein fusions with your protein of interest. See an overview of Addgene fluorescent protein...

  5. Antibody Guide

    Type
    Collection
    ...protein of interest. Signal strength is determined by how many antibodies bind the protein of interest, while... antibody of interest to a second, independent antibody for the same protein of interest, with no overlapping...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called... of the animal’s immune system. The antigen of interest is introduced to the animal, which then mounts...primary antibody which is bound to your protein of interest. Secondary antibodies can be isotype-specific ...recognize an available epitope on your protein of interest. Polyclonal antibodies are typically collected...Western blots should be run with your protein of interest and at least one ubiquitously expressed protein...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... of interest fused to the N-terminus of Amber Amber C1 Yellow Mammalian Express a gene of interest fused...a fluorescent fusion protein with your gene of interest and the listed fluorescent protein. Plasmid Color... mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean ... mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean ...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow...Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the N-terminus of monomeric Venus mVenus...mVenus C1 Yellow Mammalian Express a gene of interest fused to the C-terminus of monomeric Venus pCEP4YPet-MAMM...

  7. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...backbone can be repeated to ligate your gene of interest after the promoter, and so on. The following table... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Experimental Tips Choosing a tet system If your gene of interest should be active, and only turned off occasionally...off or tTA is more appropriate. If your gene of interest should be mostly inactive, and only turned on ...tetracycline-dependent promoter upstream of your gene of interest tTA expression plasmid Components for a tetracycline...tetracycline-dependent promoter upstream of your gene of interest rtTA or TetR expression plasmid Stable cell lines...will allow you to insert and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA... 16542 pBI-MCS-EGFP Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional...plasmid for inducible expression of transgene of interest and EGFP None Either Lung 11662 pPRIME-TET-GFP-FF3...

  9. Plan Your Experiment

    Type
    Collection
    ...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested with...region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...necessary to model your specific disease or process of interest. Do you want to: Generate complete and permanent...the promoter driving expression of your gene of interest. For genetic knockouts, gRNAs commonly target ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ... within a mixed cell population. The region of interest is PCR amplified, PCR products are denatured-renatured...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...

  10. Cre-lox system

    Type
    Collection
    ...can precisely control expression of your gene of interest. Many Cre constructs also contain fluorescent ...a promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547...-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression in the absence of... Switch: These constructs contain two genes of interest, Genes A and B. When Cre is absent, only Gene ...and type of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool...information. Empty backbones to regulate your gene of interest: ID Plasmid Description Expression System PI 11578...

  11. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Express gene of interest from the CMV promoter pCDH-EF1 72266 Express gene of interest from the EF-1 promoter... Express gene of interest from the CB promoter pCDH-PGK 72268 xpress gene of interest from the PGK (phosphoglycerate...Express gene of interest from the CB promoter pCDH-EF1s 72484 Express gene of interest from a truncated...respectively, and empty vectors to express your gene of interest under the control of a variety of promoters. Professor...kinase I) promoter pCDH-CMV4 72284 Express gene of interest from the CMV promoter. WPRE has been removed to...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple...allow you to test the function of your gene of interest in a controlled environment under various conditions... C-terminal 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc...an N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression...cell lines in which the expression cassette of interest is incorporated into the host DNA. For mammalian...you can clone your regulatory element or gene of interest to create a luciferase reporter Promoter Measure...HA or myc-BioID2-MCS - To fuse your protein of interest to the N-terminus or C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...well as plasmids that already contain a region of interest before or after luciferase . In addition, Addgene... pGL3-LRR : Genomic tiles spanning a region of interest are cloned into a Firefly luciferase reporter ...you can clone your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise...Lentiviral expression of firefly luciferase and gene of interest Bryan Welm 18760 MSCV IRES Luciferase Firefly ...Retroviral expression of firefly luciferase and gene of interest Scott Lowe 18782 MSCV Luciferase PGK-hygro Firefly...expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the plasmid. Malcolm...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...

  14. Genetic Code Expansion

    Type
    Collection
    ...acids (ncAAs). A plasmid containing the gene of interest with the modified codon (typically the amber codon...a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which is generally...codons, this can be challenging. In E.coli , the rarest codon is the amber stop codon (UAG) and thus this...this codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon ...pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest 209738 pAB228i16 G1 PylRS Methanogenic archaeon...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...

  15. Open Enzyme Collection

    Type
    Collection
    ...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... incorporation phosphoserine into your gene of interest....choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030 origins or a low copy p15a...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated...identify candidate human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ... The following resources may be of interest for groups interesting in implementing intersectional experimental...strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing...testing of novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online...

  18. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000 U/ml)...for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after ... construct. Transfecting CRISPRs into Cells of Interest NOTE: This protocol involves the delivery of CRISPR...

  19. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast.... overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization of some of...tagging vectors to create fusions of proteins of interest with yeast codon-optimized fluorescent proteins...
Showing: 1 - 20 of 65 results