We narrowed to 570 results for: SHA

1. Common Injection Routes in Mice

   Type

   Blog Post

   ... extra prep, as the injection site area must be shaved first. Restraint: anesthesia Location: between ...

2. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   ...protocols Viral Plasmids and Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002...

3. Antibodies 101: Flow Compensation

   Type

   Blog Post

   ...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...

4. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   ...the effects of CO2 on sample pH Cell morphology: Shape and size matter You should have a light microscope...

5. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   ...those markers are identical and completely indistinguishable. This tool is of particular value when designing...

6. PhD Applications After COVID

   Type

   Blog Post

   ...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   ...do we make sure to catch all of these potential mishaps before distributing the vectors? Viral genome sequencing...

8. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   ...obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully...

9. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ..., Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture of Chromatin Interactions...

10. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    ... in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since ...

11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ...the common antibody is heftier (~150 kDa) and Y-shaped because it contains two large heavy chains and ...

12. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    ...26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering via...

13. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    ...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

14. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ...through experience and from my mistakes. Here I’ll share what I wish I knew when I first became a manager...

15. Academic vs. Industry Postdocs

    Type

    Blog Post

    ...both sides of the postdoc experience, I’m going to share what I’ve learned and hopefully make your decision...

16. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    ...therapeutically due to their unusually “smooth” shape and lack of “druggable” domains. In other words,...

17. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...sequencing results or comments on our suggestions? Share your thoughts here to help other labs speed up their...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with Endy more ...

19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

20. Getting to Know Expansion Microscopy

    Type

    Blog Post

    ... actin filaments or microtubules. This issue is shared by other SRM techniques and can be alleviated by...