We narrowed to 660 results for: SIM;

1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   ...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans...

2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...expected locations. An analysis of mWasabi gave similar results. We hope this blog post helped you see ...

3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   ...the stochastic expression of XFPs is possible by simply introducing a mixture of XFP genes. Stochastic ...

4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...reporter. Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive...

5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...Wanisch  Listen to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group...

6. Science Careers: Unruly Interests Feed Many Paths

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   ...career Developmental biology caught me early on: a simple egg is packed with information. I am fascinated...

7. Using Addgene's New Viral Service for Your Research

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   ... phenotype of interest. Optogenetic tools for similarly controlling neuronal activity with light will ...

8. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ... protein (MBP, New England BioLabs). MBP has a similar function as GST in that it provides a method to...

9. The Strength of Story Telling

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   ...which makes honing your ability to tell stories a similarly crucial skill for career advancement. Scientists...

10. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...reference sequence database. Through a series of simulations and machine learning, they created a classifier...

11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...

12. Another Pathway into Cells: iTOP

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    ...instead of supplying a DNA construct, they could simply induce cells to take up protein directly? It’s ...

13. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV...

14. Summer SciComm Series: A PhD in Science Communication

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    ...adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...

15. Plasmids 101: Gram Negative and Positive Bacteria

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    ...thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear with...

16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...diseases with real phenotypic effect.” “It’s a very simple technology,” Doudna further explained. “That’s ...

17. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

18. "Build Your Plasmid": The Game - Play to Teach

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    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...

19. K. phaffii: Rising to the Occasion

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    ...evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...

20. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    ...or in cell nuclei. The two photosensitizers had similar rates of off-target damage to neighboring cells...