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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 618 results for: Set

Showing: 481 - 500 of 618 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...re-administration but I do not think the field has settled on ‘best practices’ for model organisms.    Q11...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

  2. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses... own promoter   Kabadi et al Csy4-Cleavable Cassettes transfection gRNAs expressed from a polycistronic...co-transfection of Cas9 Nissim et alTsai et al PTG Cassettes transfection gRNAs expressed from a polycistronic...

  3. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential after development... gene was disrupted and silenced by a Lox-Stop cassette, resulting in the neurological deficits observed...Restoring the MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted ...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...and deposit individual documents or more complex datasets into the desired repository. They also support...experiment documented in RSpace using the RSpace datasets deposited in Figshare or Dataverse has access ...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of tRNAs that...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...courtesy of IPI. IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies... that bound an amino acid region displayed by a subset of the RGD-binding integrins containing the αV ...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...responses to viral infections have resulted in the onset of cancer. Similar patterns of cancer development...inflammatory bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

  10. Plan Your Experiment

    Type
    Guide
    ... you started using CRISPR in your research and setting up CRISPR experiments. We will focus on making ...DNA to serve as template for the repair. Once you settle on your gRNA, you can design a donor DNA to have...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...

  11. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

  13. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... (a specific mutation in a known gene = disease onset) may be best served by this type of targeted design...hits’ - genes that affect disease progression or onset. These hits can reveal individual genes or sometimes...

  14. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

  18. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...re building software will help you be a valuable asset to the scientists as well as the developers. Lastly...

  20. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...
Showing: 481 - 500 of 618 results