Addgene

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Showing: 481 - 500 of 562 results

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CRISPR Activation: A Practical Guide

Type

Blog Post

...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

...non-cytosolic protein functions properly? We suggest starting by using the newly published oxidative optimized...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...study is an excellent example in which the authors started with a brain region that was known to be generally...
Plasmids 101: Control Plasmids

Type

Blog Post

...least you should repeat your whole experiment from start to finish multiple times and on different days. ...
Five Popular Model Organisms

Type

Blog Post

...out there. The house mouse (Mus musculus) Let’s start with the most preferred mammalian model organism...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...compared to CRISPR editing CRISPR, of course, has started a revolution in gene editing. However, while CRISPR...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...product is digested with DpnI to remove residual starting template. After transformation and harvest, the...
Five Popular Model Organisms, Part 2

Type

Blog Post

...check out Addgene’s plasmid collections to help start your experiments! References: Blount, Z. D. (2015...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...and effective in the retina of a mouse model of Stargardt disease. Hum Mol Genet 24:6811–6825 . https://...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...characterization. Figure 2: Phage display starts with a pooled library of scFvs that are displayed...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...validate it for your particular setup. We recommend starting by searching the literature to see what is already...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...options for potassium biosensors and are a promising starting point for further engineering. Figure 5: Structure-guided...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

...yourself up for a screening success? Before you start analyzing individual clones, it’s wise to quickly...
Evolution of Lab Techniques

Type

Blog Post

... interest to create a physical map of the DNA. Starting in the 1970s, restriction mapping was commonly...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...weaknesses of the options. Use this blog as a guide. Start a document to record notes and observations so you...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

...research enterprise time and funds. It’s time to start putting them into practice! Additional Resources...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

...doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

..., we suggest these serotypes as a good place to start. Table 2: AAV tissue tropism for mice in vivo. ...
Quick Guide to All Things Lentivirus

Type

Blog Post

...well. Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...dsDNA-dependent exonuclease. Exo will degrade linear dsDNA starting from the 5’ end and generate 2 possible products...

Showing: 481 - 500 of 562 results

Results per page:

CRISPR Activation: A Practical Guide

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: Control Plasmids

Five Popular Model Organisms

Adeno-associated Viruses (AAVs) for Genome Editing

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Five Popular Model Organisms, Part 2

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Antibodies 101: Single Chain Fragment Variables (scFvs)

Antibodies 101: Normalization and Loading Controls for Western Blots

Hot Biosensors 2022: Year-End Roundup

CRISPR 101: Validating Your Genome Edit

Evolution of Lab Techniques

Is this the right place for me? 8 tactics for choosing a lab

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Antibodies 101: The Basics of Western Blotting

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Quick Guide to All Things Lentivirus

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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