We narrowed to 617 results for: TIL

1. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   ...core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) since 2005...

2. Antibodies 101: Fab Fragments

   Type

   Blog Post

   ...resulting product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - ...

3. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   ...begin, which all takes time. Though the time delay until expression is highly dependent on the serotype and...

4. All in a Twist: dsRNA

   Type

   Blog Post

   ...stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store their genetic information...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ...what if you just want to change protein levels? Until recently, no such tool existed for rapid, customizable...

6. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...2018). Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents...

7. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ...informed discussion, debate, and data collection still remain: What are the current numbers of trainees...

8. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   ...effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such that custom...

9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...to work with than regulatory proteins. There are still things we haven’t solved that stop it from being...

10. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    ...making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is ...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    ...“flanked by loxP”), will permit gene expression until Cre is present, at which time the gene will be disrupted...

12. Working with Nuclear Receptors

    Type

    Blog Post

    ...maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction. Both...

13. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ...auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans." ...

14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    ...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...

15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering...

16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    ...you can make big changes in your career path and still find comfort and happiness there. Read all of the...

17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    ...targeting the same cell class in all three species are still greater than random chance. The chances of a promoter...

18. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    ... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating...

19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent...

20. Design Tips for Prime Editing

    Type

    Blog Post

    ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different...