We narrowed to 630 results for: TIL;
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TypeBlog Post...stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene. Yen et...
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All in a Twist: dsRNA
TypeBlog Post...stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store their genetic information... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...Raf-MEK-ERK signaling pathway to demonstrate the utility of photoswitchable kinases in vivo with C. elegans... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post... (re-ligated empty vectors, for instance) would still express ccdB and therefore would die. This procedure... -
The Future of Research Symposium Boston 2015
TypeBlog Post...informed discussion, debate, and data collection still remain: What are the current numbers of trainees... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such that custom... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...to work with than regulatory proteins. There are still things we haven’t solved that stop it from being... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is ... -
Plasmids 101: Cre-lox
TypeBlog Post...“flanked by loxP”), will permit gene expression until Cre is present, at which time the gene will be disrupted... -
Working with Nuclear Receptors
TypeBlog Post...maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction. Both... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post... innovation by allowing scientists to find and utilize reliable, high-quality antibodies. We are creating... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...GFP, our most-requested GFP(1–10) and GFP(11) Versatile protein tagging in cells with split fluorescent... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering... -
Design Tips for Prime Editing
TypeBlog Post...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...you can make big changes in your career path and still find comfort and happiness there. Read all of the... -
Hot Plasmids February 2024
TypeBlog Post...acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a recombinant... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...they retain their intracellular interactions and still set off the same pathways, giving rise to the same... -
Viral Vectors 101: Virus Safety
TypeBlog Post...cycle once inside the host. While the virus can still enter (and thus infect) cells, this safety feature... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...toolbox with CRY2clust Article contributed by Brook Pyhtila Listen to the CRY2clust podcast segment The Won...