...creativity and expertise of the group and yet never hesitated to reach out to the greater research community...portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus...and the School of Molecular Sciences at Arizona State University. His group carries out research in synthetic...

... the NIH, started off with the first keynote presentation. He talked about the impact of the Human Genome...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...species for genome engineering. The final keynote presentation was by Nobel Laureate Thomas Steitz of Yale....

...difficult person: 1) Understand your supervisor’s expectations Every supervisor has their own management style...frequently. The easiest way to meet your supervisor’s expectations is to ask upfront: “How frequently would you... details of your work. Describe the problem by stating the facts and explain how it is interfering with...

...genetically modified organisms, and GMOs have a bad reputation. The public is can be fearful of this technology...ended up applying to a business accelerator freely stating that we had an idea, knew how to build it, but ...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

... clearly As a manager you need to clearly state expectations and goals. Yours and your team’s success ... schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

...like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The most common production...immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (Aurnhammer et al., 2012), droplet...what Addgene uses, check out our ddPCR for AAV quantitation blog post.  We generally do not recommend using...

...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids requested, categorized...total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that centers around the impact...

...proteins that can be easily modified in order to facilitate its entry into the target cells (Beatty et al...tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports paper introducing... (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow 81...

... of many cellular mechanisms, there are some limitations to working with FPs. Overexpression of fluorescently...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...However, CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput...

...this process accounts for a percentage of the mutations seen with TALENs and CRISPR. MMEJ is active during...using much smaller regions of homology, which facilitates easier cloning. An overview of PITCh. ...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular...genes from organisms with high CpG dinucleotide representation. The specific restriction enzymes used in various... 3. The goal of high throughput cloning is to facilitate the construction of plasmids with many components...

...nothing’s ever perfect, neither is AAV. Its main limitation is the small size of its DNA cargo - 4.8 kb at...strategies are being optimized to overcome this limitation, with limited success so far. Luckily for patients...an ever-growing number of clinical trials for devastating genetic diseases such as hemophilia, muscular...

...attend? What is the expectation on level of critique and detail? Who is/are the facilitator(s) of the journal...papers could be more important.  Any slides, presentations, recordings, and other materials should be made...

...protein synthesis. The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it....when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA during selection if it...good choice for experiments in which off-target mutations are undesirable. Pros: Works across many species...

...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some of the ...doi.org/10.1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy...

...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive RNA-...hits in genetic screens, they do have several limitations. CRISPRko results in a complete loss of function...

...the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since we...simultaneously allowing scientists to actively comment, annotate, and edit protocols. After my introduction to ...lab-use tablet? The software also makes it easy to annotate protocols, make them public for others to use,...

...acceptor FRET know-how with aptamers, aptabeacons (or aptatags) were born. Aptabeacons can be created in many...general premise is that an aptamer in its unbound state contains a fluorophore and a quencher in close proximity...brighter version of the aptamer, but it still had limitations in vivo. Using a combination of SELEX and fluorescence-activated...

.... In vivo, these recombination reactions are facilitated by the recombination of attachment sites from...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry vector.... to 4 DNA fragments, in a specific order and orientation, in one tube, into one Gateway vector to produce...

...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...individuals, asbestos has not been banned in the United States and many other countries, and it is still imported...