...reference library. If this happens to you, one solution is to identify all the alternative names for your...screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular biology 370.3...

...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...than an accessible/inaccessible binary. A word of caution: CGBEs and indels Cytosine transversion editors...

...Aug;10(2):302-17. PubMed . From virus evolution to vector revolution: use of naturally occurring serotypes...thus thought to be the most suitable virus for therapeutic applications. Clinical trials using AAV for various...gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors for enhanced...

...methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy...and methanol, we found that the optimal perm/fix solution depended on the specific antibody:target combination...

...transfer plasmid. 2nd generation lentiviral plasmids utilize the viral LTR promoter for gene expression, whereas...whereas 3rd-generation transfer plasmids utilize a hybrid LTR promoter (more information on this below)...office can provide more information on your institution's best practices with regard to lentiviral research...generation vectors use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates...

...vivo and the receptors exhibited high levels of constitutive activity, making them less than ideal. DREADDs...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have ...This DREADD was shown to have a small amount of constitutive activity, and is not widely used. There is also...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be...

... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute ..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...

...excited to share some good news with you: with contributions from depositors around the globe, we’re now ...

...easily access relevant information for planning, executing, and troubleshooting your experiments. For example...

...contributed to the Addgene blog this year! Your contributions have helped researchers around the globe find...

...and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the new system...

...) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ...

...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...

...the data hub website as a thank you for your contribution to open science.  Please note that the AAV Data...

...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); ...genetic knockouts, gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances ...

...Great Depositor Research Stories Interested in contributing to the Addgene blog? We'd love to hear from ...

...Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources on Addgene.org Use...

... a colleague who would benefit from our free distribution services for their popular constructs, why not...

...hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used at their lab benches...