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We narrowed to 771 results for: Vars

Showing: 481 - 500 of 771 results

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  7. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...researcher and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze...be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction efficiency...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

  10. Hot Plasmids - October 2020

    Type
    Blog Post
    ...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ..., FLEx is currently being used to study a wide variety of in vivo conditional gene rescue experiments,...switches to conditionally test the function of various point mutations and truncations in the absence ...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...share plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized...identify. There were dozens of different mushroom varieties alone. At least I could tell that they were mushrooms...

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...have been discovered to play a large role in a variety of diseases, ranging from cancer, to diabetes, ...transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015): 533-537. ...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous...assay dozens of GPCRs across many cell types in varying conditions, revealing context-dependent responses...

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...factors. You have to weigh the pros and cons of various methods and choose what is best for you — and the...array of purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...protein production. Titration of baculovirus can vary depending on the protocol used. Plaque assays are...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...destabilizing domains which will be identified by various enzymes and sent to the proteasome for degradation...protein levels aren’t limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate...
Showing: 481 - 500 of 771 results