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  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  2. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  5. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...

  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...flexible nature of MoClo allows users to test a variety of different orientations and components within... allows researchers to assemble and test many variables all at once. CRISPR CRISPR arrays and libraries...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... either 3-base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    ...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...researcher and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze...be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction efficiency...
Showing: 481 - 500 of 767 results