...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...

... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ... Various Addgenies...

...several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be ...SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out any biochemical...hundreds of additional plasmids available through Addgene, for study of the of WW, PDZ, and other protein... Various Addgenies...

...scientific sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools...29551272 Plasmids from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact ...29551514 Plasmids from this paper are available at Addgene. Learn more about research at Arbor Biotechnologies...Biotechnologies. Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips...Cas13a (C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR...

...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

...have already been built and are available on the Addgene catalog – so check there before building one from...therapy—viruses could further your efforts. And Addgene is here to help! We offer a variety of viral vectors... Click Here to Find Viral Vector Resources at Addgene! References and Resources References Travieso...s41392-021-00487-6 Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...

... More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide   ...transduction. Most transfer plasmids available from Addgene were derived from MoMLV (Moloney Murine Leukemia...im1017cs31. PMID: 18432682. More resources on the Addgene blog Viral Vector Elements The Retroviral Life ...This post was written and updated by multiple Addgenies. It was originally published in 2018, in the Viral...

...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly - which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors...discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine...

...plasmids or plant expression systems available from Addgene. If you have any questions or thoughts about this...PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed....  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI nucleases for..... Find the plasmids from this publication at Addgene ...

...sections of the current NURSA website. NURSA and Addgene The great progress that has been made in the NR...literature and acquiring them in a timely manner. Addgene represents an excellent solution to the problem...forward to growing this important relationship with Addgene for the benefit of the entire research community...STKE. 2005, tr22. Keep on reading! Check out Addgene's Plasmids 101 blog series. ...

...this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot chocolate...

...plants available through Addgene. In addition to the plasmids described above, Addgene carries several useful... are available to academic researchers through Addgene. CRISPR components for plant genome engineering...expression of small RNAs in their respective species. Addgene carries >30 “empty gRNA” backbones which contain... or ubiquitin promoters (Belhaj et al., 2013). Addgene carries Cas9-containing plasmids for knockout, ...s41580-020-00288-9 Additional Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate...Edit Design Your gRNA Additional Resources on Addgene.org Find CRISPR Plasmids for Plants Read Our CRISPR...

...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...between 1 to 2 kb in length. There are plenty of Addgene plasmid options for templates - see the example...construct as described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below...How to design Easi-CRISPR repair templates using Addgene plasmids. Design PCR primers to add T7 promoter-left... PMCID: PMC5828090 Additional Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models...Ribonucleoprotein (RNP) Delivery Resources on Addgene.org Check out our CRISPR Guide Find Plasmids for...

...of the Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page...PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano...PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo &...PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and expanded Q-system...PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile...PubMed PMID: 22129153. Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources... will review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too. The AspFlex... (Brychcy et al., 2023). For plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits...study in yeast cells. Need more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs ... resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden Gate Cloning ...

...If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you...placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the... we’ll take a deeper dive into the content of Addgene’s aptamer soup by exploring the development and ...and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach...PMCID: PMC4143336. Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate...Find other uses for RNA aptamers Resources at Addgene.org Browse fluorescent protein plasmids and resources...