We narrowed to 952 results for: cha;

... Joanna Brownstein (our outgoing Chair) and Dave Root (incoming Chair) and our entire supportive Board...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...

...scientists communicate with each other: from formal mechanisms like publishing a research article, presenting...settings like lab meetings, social media, coffee chats with potential collaborators, blogging and more....read freely by anyone and are typically free of charge for authors to post. They can be assessed and commented...authors over time, providing a public record of changes, critiques or endorsements. In contrast, journal-based...

...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...coupling can be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically...that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies...References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler’s guide to cytometry...

...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called...between the non-target strand and a positively charged groove formed by the Cas9 HNH and RuvC nuclease...al reasoned that, if they decreased the positive charge in the HNH/RuvC groove, then they could weaken ...interactions between the groove and the negatively charged DNA and therefore destabilize basal strand separation...

... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...shape of the response function or how the output changes as a function of input. Usually that function is...enough genetic parts for complexity. We have characterized almost 600 terminators. That gives us enough... we’ve really been making tons of parts and characterizing the software to help you put it together. The...

...Indicate the amino acid change as well as any functional consequences of that change. For example, “Mutated... Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...cell types and for viewing how gene expression changes through time or after genetic manipulation. The...tissue sample, several washes, and temperature changes to visualize the expression of specific genes. ...genes of interest and was first discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering...

...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...to present about your prior research. This is a chance to highlight your prior accomplishments and demonstrate...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...their hand to ask a question or type it into the chat. Either way, just let the interviewers know up front...

...light-activated ion channels. Similarly, chemogenetic tools are designed channels that are chemically ...introduced in neurons and depending on the choice of channel protein, they can trigger neuronal excitation or...adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed PMID: 23686825. PubMed Central...

...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one...306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination ...

...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions.... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It... action items. This will give your supervisor a chance to review what you have discussed and add suggestions...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

...genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In fact, the... software. References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations....2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods for Quality Control Testing of Recombinant...

... 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning... to recover after they are passaged. This could change their growth rates and morphology, rendering their...numbers Keep an eye on the passage number. Cells change their growth rate and morphology at high passage...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, V., Bosch, F., Bose, M., ...., Francis, J. D., … Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type...

...mGold2t (Lee & Lai, et al., 2025). The team characterized and deposited a variety of constructs for visualizing...perform neuronal tracing, anterograde tracing poses challenges, from non-selective anterograde transport to ...transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based...or delivered separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag...

...AAV-containing 40% fraction is collected and buffer exchanged to remove the iodixanol and concentrate the purified...or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the AAV Iodixanol Gradient...correctly. When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots...vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: 18265393. Additional...

...through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated by natural...previous gene drive and thereby undo the genetic changes driven by the earlier intervention2. While an initial.... PubMed. Gantz, V. M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous to...10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M...

...purify proteins can face any number of technical challenges depending on their protein of interest. After...After several decades of trying to address these challenges, researchers have amassed a considerable molecular...for your protein. If you’re working with an uncharacterized protein, or a protein for which a good antibody...KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance of charged and polar residues improves solubility; good for...

...effects. The flaws of RNAi screens are well characterized and strategies exist to control for these faults...also reproduced several previously observed characteristics that influence gRNA off-target activity: 1)...23394947. Pubmed Central PMCID: 4299491. 5. Bassik, Michael C., et al. "A systematic mammalian genetic interaction...available from Addgene Other Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens...

...of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work...viral vectors are preferred. This is to avoid the chances of insertional mutagenesis, which is the integration...replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the enhancement of iPSC generation...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...