We narrowed to 636 results for: erf

1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ... Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research...

2. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...quite small—20 amino acids—so we don’t think it interferes with the function of the protein. Myers: We’ve...

3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...treatment with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang...

4. CRISPRainbow and Genome Visualization

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   ...BioRender.com. With this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal...

5. Troubleshooting Your Plasmid Cloning Experiment

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   ...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...

6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...state of the photoswitchable proteins offers a powerful approach to control protein-protein interactions...

7. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify...

8. Antibodies 101: Affinity Reagents

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   ...exciting time to be a scientist looking for that perfect fit!  References and Resources Additional resources...

9. Antibodies 101: Selecting the Right Antibody

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   ...Antibody-based assays are complex and antibodies may perform differently in different samples and experimental...

10. The time and cost required to make a plasmid

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    ...the time spent by the scientists designing and performing the experiments. This cost varies hugely based...

11. Plasmids 101: The protein expression toolbox

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    ...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ...

12. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism of...

13. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...modification(s) Congratulations! You’ve picked your perfect guide; now check out our blog posts on choosing...

14. Antibodies 101: Polyclonal Antibodies

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    ...immunogen. A different type of purification can be performed to isolate antibodies only recognizing your protein...

15. RNA Extraction Without A Kit

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    ... to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as stable...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...CreLite-mediated recombination was found in both deep and superficial tissues of zebrafish studied, such as the brain...

17. Celebrating 15 Years of Scientific Sharing

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    ... At Addgene we will never ever stop planning, perfecting, and learning, but there are a lot of things ...

18. dTAG - You're it!

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    ...dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute selectivity...

19. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...

20. Plasmids 101: Golden Gate Cloning

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    ...exciting applications, this system is an incredibly powerful tool for cloning complicated constructs in a single...