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Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

...suggests new strategies to manipulate that process. We began our research with a simple question: how do Cas9...
Addgene Welcomes John Doench to the Advisory Board

Type

Blog Post

...they do and what they might be able to do and to organize it and catalogue it, I mean that’s the critical...
Choosing Your Fluorescent Proteins for Multi-Color Imaging

Type

Blog Post

...no single fluorescent protein optimal for all organisms and that if you want the brightest signal, you...
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Type

Blog Post

...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

...Bench setup Small adjustments in the physical organization of your workspace and your workflow can have...
New and Upcoming Viral Vectors - May 2020

Type

Blog Post

...lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Type

Blog Post

... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...basics. Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...
Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

... the different website navigation paths, the organization and completeness of our information, and the...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...the OptoNB could bind and block a receptor or a ligand’s activity, then a flip of a light switch could ...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Type

Blog Post

...graduated with highest honors from the University of Michigan, Ann Arbor and obtained her J.D. from Boston University...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...
Viral Vectors 101: Biosensors

Type

Blog Post

...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...
Viral Vectors 101: Types of viruses

Type

Blog Post

...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...
From Lab Bench to Leadership: Transitioning into Management as a PhD

Type

Blog Post

... in the wet lab wasn’t the right path for me. I began exploring alternative career trajectories within...
Addgene’s Viral Service - Why Virus? Why Now?

Type

Blog Post

... would like to thank some of the people and organizations who helped us reach this milestone. Viral service...
Clear and Accessible Writing

Type

Blog Post

... As we incorporated alt text into our work, we began to realize how the principles we used in our daily...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...

Showing: 481 - 500 of 607 results

Results per page:

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Addgene Welcomes John Doench to the Advisory Board

Choosing Your Fluorescent Proteins for Multi-Color Imaging

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Sweating the Small Stuff: Details in the Lab

New and Upcoming Viral Vectors - May 2020

Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Developing a cold tolerant E. coli using specialized chaperones

Behind the scenes: Addgene’s new search engine and more

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Biosensors

Viral Vectors 101: Types of viruses

Sharing is Caring: But How to Distribute Open Hardware?

From Lab Bench to Leadership: Transitioning into Management as a PhD

Addgene’s Viral Service - Why Virus? Why Now?

Clear and Accessible Writing

CasPEDIA: A Functional Classification of Cas Enzymes

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