We narrowed to 537 results for: invs

1. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP...

2. CRISPR-based Genome Editing Systems For Editing in E. coli

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   Blog Post

   ... Image from Li et al., 2021.   The updates involved the following changes. In pCas9, we 1) replaced...

3. Hot Plasmids - August 2020

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   Blog Post

   ... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

4. Plasmids 101: Plasmid Incompatibility

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   Blog Post

   ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...

5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   Blog Post

   ...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

6. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

7. CRISPR-mediated Plant Base Editors

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   Blog Post

   ... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

8. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   Blog Post

   ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

9. To Codon Optimize or Not: That is the Question

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   Blog Post

   ...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

10. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...who has gone where you hope to go, and who will invest in your success. If you are a graduate student ...

11. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...we do know that there are some genetic factors involved. For example, multiple studies show that certain...

12. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...essentially we just needed to send a Google calendar invite over to the r/Science team with a description of...

13. Tips for CRISPR Gene Editing in Mice

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    Blog Post

    ...supplied by the Zhang lab. This simple website invites you to input the target region (approximately 500bp...

14. Tips for Using FRET in Your Experiments

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    Blog Post

    ... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...

15. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post

    ...before others. In some cases, typically consortia involving genomic screens, the data stream is so prolific...

16. Hot Plasmids: Summer 2024

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    Blog Post

    ...Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing...

17. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

18. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ...These Opto-RhoGEFs provide a reversible and non-invasive way to activate a RhoGTPase by recruiting its ...

19. Tips for Getting a Faculty Position

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    Blog Post

    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...

20. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved...