We narrowed to 997 results for: lars;

...from purified recombinant proteins at small and large scale.  This new technique involves selection in...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...affinity purification required! The team used other molecular techniques to introduce time modulation of the... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...PHP.V1 that exhibits efficient transduction of vesicular brain cells. Find this AAV at Addgene New serotypes...

...their communication with gene promoters. Cellular and Molecular Life Sciences, 78(19–20), 6453–6485. https...systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX...investigator at the Allen Institute in the department of Molecular Genetics. References and Resources References...Zhou, T., Mortrud, M., Yao, S., Siverts, L. A., Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., ...

..., which is not well protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs...related voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ... did, and state that it would help you manage a large number of files as part of the job. Structure of.... It can also convey your excitement for that particular job, your commitment to the organization's mission...

...complex approaches to scientific questions become popular. There are only so many methods and machines one...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... talking to them at a seminars, or even set up regular meetings. Meeting people outside your organization...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more...young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...getting a phone interview! If you don’t have a particular employer in mind, you may consider starting with...pages. Make yourself visible on LinkedIn and other popular job site webpages. Importantly, find a good mentor...

... pCas/pTargetF system has been one of the most popular genomic editing systems in E. coli. However, some... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated...Academy of Sciences (CAS) Center for Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences since December 2020. His research... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate...

...restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given...1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA...has ready-made entry clones available for many popular genes, including Hs.KRAS4a. Use our website to ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

...often used to study the biology surrounding a particular gene (although it has been quite successfully...mutation and need to focus your attention to that particular region. No matter where you are looking, you ...the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression), one gRNA at a time...Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...

...research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...test chamber is used in which a nose-poke at a particular hole (the active hole) induces laser stimulation...without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation and is a sufficient... happens. You never know how stimulation of a particular brain region may affect the animal; stimulation...

...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...arms between 500-1000 bps. HDR efficiency with circular plasmid templates is generally low; to increase...cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed. The Road Less ...

...landmark discovery in the 1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker...blog, we’ll be focusing on shRNA, one of the most popular choices for gene silencing. RNA interference RNAi...degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/10.1016/j.... and assessment of gene knockdown. Methods in Molecular Biology, 139–156. https://doi.org/10.1007/978-...on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology Reference ...

...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

...Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, harnessed these attributes of ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...as connections between motor neurons and the neuromuscular junction. In zebrafish, the method has become...neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods...

...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...cumate-inducible gene expression system, which functions in a similar manner to the Tet system. The transactivator configuration...interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed... binary systems above if they all behave very similarly?” The simple answer is that sometimes it is necessary...systems. Recent work has even used all 3 in a single modular vector for ease of use! Conclusion This post contains...

...hard work and talent for many years (at a very low salary I might add). You have a right and responsibility...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep... and learn. Graduate students and postdocs in particular must care about this. The training you get now...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

...plasmid molar ratio you would like to use for your AAV packaging transfection. In my lab we regularly use ...requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do... Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID: 18265393. ...

...Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

...Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions between crops like...around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning...plasmids he acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase...heavy focus on teaching. He hopes to teach his molecular biology students about the functions of these ...

... you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery rate... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...positives/negatives, but they all generally yield a similar result and are appropriate to use if all sample...