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We narrowed to 810 results for: mal

Showing: 481 - 500 of 810 results
  1. Celebrate National Book Month with Addgene

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    Blog Post
    ...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...
  2. Hot Plasmids - January 2023

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    Blog Post
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...
  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...and has extensive experience targeting RNA with small molecules. Other members’ projects range from hacking...Ph.D. grad student at Boston University studying mammalian immunology, and in his free time, he is pursuing...
  4. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...
  5. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ...PAM-distal side of the break and 91bp on the PAM-proximal side, consistently supported higher frequencies...attained without any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know ...
  6. Components of CRISPR/Cas9

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    Blog Post
    ...or closely related Cpf1) to cut the genome and a small RNA (gRNA) to guide this nuclease to a user-defined...used for CRISPR-mediated genome editing, these two small RNAs have been condensed into one RNA sequence known...
  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...create GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did ...
  8. Plasmids 101: Inducible Promoters

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    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters...
  9. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ... attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular... which can then be exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al....
  10. Fluorescent Proteins 101: Luciferases

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    Blog Post
    ...Luciferase is the best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris... they emit or to optimize their expression in mammalian systems. Our luciferase collection page has a ...
  11. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ... replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...preceded by GA or followed by TC) would be cut as normal. If not taken into account, this blocked site could...
  12. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...
  13. Antibodies 101: Fab Fragments

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    Blog Post
    ...resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa.  Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently...
  14. Plasmid Tools for Microbiome Studies

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    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...
  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...
Showing: 481 - 500 of 810 results