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We narrowed to 850 results for: met

Showing: 481 - 500 of 850 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...many cases fluorescent proteins have usurped old methods or complemented them in ways that make them much... now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of...FP-timer protein or protein fusion. This one protein method simplifies cloning and makes your experimental ...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...decision a little easier if an industry postdoc is something that you’re considering. Like anything, there ...can’t afford to spend too much time working on something extremely risky or exploratory. Getting a job ...specific web sites for postdoc listings, so the method of posting varies between companies. Like most ...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...laboratory populations with that sequence2. Both methods are easy to implement and have been tested in yeast5...can't be performed with two or more confinement methods. Since molecular, ecological, and reproductive .... M. & Bier, E. The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...interest, scientists have instead developed novel methods to co-express your gene along with a reporter, ...the genes expressed from the same cassette is sometimes advantageous, particularly when only a portion...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

  5. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...animal sacrifice. Could there be an alternative method for immunodetection? Enter the Görlich lab and ...and extracted with common protein purification methods. Read on to learn more about nanobodies and how...isolating HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several...

  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...mini-prep the positive ones. Regardless of which method you choose, make sure to use the appropriate antibiotic...amplify a product, then you know there could be something wrong with the PCR setup and/or the primer design...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... selection Another option is to use a different method of selection entirely. Auxotrophic selection relies...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...vectors themselves to be a distinct gene delivery method. We focused this post on shuttle vectors delivered...

  8. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as alkyladenine... DNA, creating an abasic site. MPG / AAG N-methylpurine DNA glycosylase / alkyladenine DNA glycosylase...editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8)...

  9. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...higher than traditional homologous recombination methods). By using mCherry for selection of sgRNA plasmid...Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...kDa) and are compatible with common microscopy methods. Plus, the luminescence wavelength (460 nm) is ...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...story. Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...contribute to this regulation in different ways: methylating newly synthesized DNA, blocking binding sites...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox...next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display higher ...

  13. How to Be an Excellent Trainee

    Type
    Blog Post
    ...understand the purpose of, or a different way of doing something then you’ve done previously) you can bring those...especially when you are in a new place, learning something new. So how can you remember everything? Write...done a technique before, but you haven’t, say something. It can be intimidating to ask for clarification...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...surface protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence...these cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...support entry of required metadata, with the option of adding additional metadata in the repository after...management apps, colony management software, and method repositories like protocols.io. These tools are...

  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to assess bARGSer expression, from left to right...produce GVs in their own lab using the most reliable method, which is the transient cotransfection of the mARG...diagram of mARGAna. (b) Schematic of the general method to create your own mARGAna cell line. (c) Z-projected...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ... assumptions, use of inappropriate statistical methods, omission of some data, etc.  The firestorm that... for increasing both data accessibility and methodological detail. Publishers have also contributed to...to this issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures...

  18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...integrate into the host cell’s genome. However, sometimes inte-great-ion isn’t so great. In this post, we...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells...piece to measure! Instead, two commonly employed methods for titering IDLVs include detecting vector particle...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components as ribonucleoproteins...separately from the viral vector. This delivery method frees up space in the AAV allowing a researcher...PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating human somatic cell gene knockouts...

  20. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...be. If people or teams can’t see eye to eye on something and a disagreement festers, it can lead to hurt...Person A one thing and inadvertently told Person B something else.") The majority of conflicts I address in...communication. Making some assumptions about how something should be communicated is necessary in a large...
Showing: 481 - 500 of 850 results